Recombinant
RabMAb

Recombinant Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free (ab236941)

Overview

  • Product name

    Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free
    See all Human IgM primary antibodies
  • Description

    Rabbit monoclonal [EPR5539-65-4] to Human IgM - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified).

  • Positive control

    • Human plasma, tonsil and spleen lysates; Human tonsil tissue
  • General notes

    ab236941 is the carrier-free version of ab134159 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236941 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5539-65-4
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Relevance

      IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
    • Cellular localization

      Cell Membrane and Secreted
    • Database links

    • Alternative names

      • Immunoglobin heavy chain constant region mu antibody
      • AGM1 antibody
      • Constant region of heavy chain of IgM antibody
      • DKFZp686I15196 antibody
      • DKFZp686I15212 antibody
      • FLJ00385 antibody
      • Ig mu chain C region antibody
      • IGHM antibody
      • IgM heavy chain constant region antibody
      • Immunoglobin heavy constant mu antibody
      • Immunoglobulin mu antibody
      • MGC104996 antibody
      • MGC52291 antibody
      • MU antibody
      • VH antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Human IgM with ab134159 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134159).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab236941 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab236941.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up