Recombinant Anti-Human IgM antibody [RM121] (ab193159)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM121] to Human IgM
- Suitable for: Sandwich ELISA, ELISA
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Human IgM antibody [RM121]
See all Human IgM primary antibodies -
Description
Rabbit monoclonal [RM121] to Human IgM -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Full length protein corresponding to Human IgM. Human IgM
Database link: P01871
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A affinity purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM121 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
Use: 50ng/well – 200ng/well (for Capture); |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. Use: 50ng/well – 200ng/well (for Capture); |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3507 Human
- Omim: 147020 Human
- SwissProt: P01871 Human
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Alternative names
- Immunoglobin heavy chain constant region mu antibody
- AGM1 antibody
- Constant region of heavy chain of IgM antibody
see all
Images
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A titer ELISA using ab193159. The plate was coated with different amounts of human IgM. A serial dilution of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA of human immunoglobulins shows ab193159 only reacted to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Datasheets and documents
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Datasheet download
References (0)
ab193159 has not yet been referenced specifically in any publications.