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    human-igm-antibody-rm121-ab193159.pdf

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Immunology Immunoglobulins Heavy Chain IgM
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Recombinant

Recombinant Anti-Human IgM antibody [RM121] (ab193159)

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ELISA - Anti-Human IgM antibody [RM121] (ab193159)
  • Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)
  • ELISA - Anti-Human IgM antibody [RM121] (ab193159)
  • Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)
  • Anti-Human IgM antibody [RM121] (ab193159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM121] to Human IgM
  • Suitable for: Sandwich ELISA, ELISA
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-Human IgM antibody [RM121]
    See all Human IgM primary antibodies
  • Description

    Rabbit monoclonal [RM121] to Human IgM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Full length protein corresponding to Human IgM. Human IgM
    Database link: P01871

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A affinity purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM121
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgM

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration.

Use: 50ng/well – 200ng/well (for Capture);
0.05μg/mL – 0.2μg/mL (for Detection);

ELISA
Use at an assay dependent concentration.
Notes
Sandwich ELISA
Use at an assay dependent concentration.

Use: 50ng/well – 200ng/well (for Capture);
0.05μg/mL – 0.2μg/mL (for Detection);

ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
  • Cellular localization

    Cell Membrane and Secreted
  • Database links

    • Entrez Gene: 3507 Human
    • Omim: 147020 Human
    • SwissProt: P01871 Human
    • Alternative names

      • Immunoglobin heavy chain constant region mu antibody
      • AGM1 antibody
      • Constant region of heavy chain of IgM antibody
      • DKFZp686I15196 antibody
      • DKFZp686I15212 antibody
      • FLJ00385 antibody
      • Ig mu chain C region antibody
      • IGHM antibody
      • IgM heavy chain constant region antibody
      • Immunoglobin heavy constant mu antibody
      • Immunoglobulin mu antibody
      • MGC104996 antibody
      • MGC52291 antibody
      • MU antibody
      • VH antibody
      see all

    Images

    • ELISA - Anti-Human IgM antibody [RM121] (ab193159)
      ELISA - Anti-Human IgM antibody [RM121] (ab193159)

      A titer ELISA using ab193159. The plate was coated with different amounts of human IgM. A serial dilution of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    • Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)
      Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)

      Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • ELISA - Anti-Human IgM antibody [RM121] (ab193159)
      ELISA - Anti-Human IgM antibody [RM121] (ab193159)

      ELISA of human immunoglobulins shows ab193159 only reacted to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    • Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)
      Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)

      Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • Anti-Human IgM antibody [RM121] (ab193159)
      Anti-Human IgM antibody [RM121] (ab193159)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab193159? Please let us know so that we can cite the reference in this datasheet.

    ab193159 has not yet been referenced specifically in any publications.

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