Overview

  • Product name

    Human IL-1 alpha ELISA Kit
    See all IL-1 alpha kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    0.82 pg/ml - 600 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.25 89% - 108%
    Serum 91.89 80% - 101%
    Plasma 92.46 82% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-1 alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-1 alpha 2 vials
    IL-1 alpha Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-1 alpha Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    IL-1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
  • Cellular localization

    Secreted
  • Alternative names

    • Hematopoietin 1
    • IL 1
    • IL 1 alpha
    • Il 1a
    • IL1
    • IL1 ALPHA
    • IL1-a
    • IL1A
    • il1alpha
    • IL1F1
    • Ilia
    • Interleukin 1 alpha
    • Interleukin 1 alpha precursor
    • interleukin 1a
    • Interleukin1 alpha
    • OTTHUMP00000203692
    • OTTMUSP00000016430
    • Preinterleukin 1
    • Pro interleukin 1
    • Pro interleukin 1 alpha
    • Prointerleukin 1
    • RP23-160G19.8
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100560

  • Representative standard curve using ab100560

Protocols

References

ab100560 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your reply.

We do not have any antibodies against your target of interest that we can guarantee will work in bovine samples. However based on the homology information I provided in the previous email, I think there is a good chance that any of our antibodies would work for you.

The below links will take you lists for all of the antibodies we have against your targets of interest.

IL-1 alpha
https://www.abcam.com/index.html?t=7587&pt=1

IL-1 beta
https://www.abcam.com/index.html?t=7588&pt=1

MMP13
https://www.abcam.com/index.html?t=5051&pt=1

MMP3
https://www.abcam.com/index.html?t=5051&pt=1

If you wish to take advantage of our testing discount program to try these antibodies in bovine samples, or if there is anything else I can help you with, then please let me know.

Read More

Answer

Thank you for contacting Abcam.
I have searched through our catalogue, and I cannot find any ELISA kits that we can guarantee will work in bovine samples, but there are a few that might work for you.
https://www.abcam.com/IL1-alpha-Human-ELISA-Kit-1-x-96-Well-Plate-ab100560.html
https://www.abcam.com/IL1-beta-Human-ELISA-Kit-1-x-96-Well-Plate-ab100562.html
https://www.abcam.com/MMP13-Human-ELISA-Kit-1-x-96-Well-Plate-ab100605.html
https://www.abcam.com/MMP3-Human-ELISA-Kit-1-x-96-Well-Plate-ab100607.html

Cross-reactivity tests with the ELISA kits listed have not been performed for bovine samples, but both forms of bovine IL-1 (alpha and beta) do exhibit a high degree of sequence homology with IL-1 gene products from mammalian species - http://www.ncbi.nlm.nih.gov/pubmed/3261832.
The MMP-3 gene is conserved in human, chimpanzee, dog, cow, mouse, rat and chicken - http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=full_report&list_uids=281309&itool=HomoloGeneMainReport.
MMP-13 has 94% sequence homology with human MMP-13 - http://www.lsbio.com/Antibodies/MMP13-Antibody-LS-A1555/48142.
since we cannot guarantee that these antibodies would work for you in bovine samples, then you could take advantage of our 100% Testing discount program:
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

If there is anything else I can help you with, or if you do wish to take advantage of the testing discount program, then please let me know.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up