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    human-il-1-alpha-elisa-kit-ab108864.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Other
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Human IL-1 alpha ELISA Kit (ab108864)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.95 pg/ml
    • Range: 0.977 pg/ml - 250 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Recombinant Human IL-1 alpha protein (His tag) (ab215012)

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    Overview

    • Product name

      Human IL-1 alpha ELISA Kit
      See all IL-1 alpha kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.7%
      Inter-assay
      Sample n Mean SD CV%
      Overall 7.1%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      > 0.95 pg/ml
    • Range

      0.977 pg/ml - 250 pg/ml
    • Recovery

      98 %

    • Assay time

      5h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s IL-1 alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 alpha concentrations in cell culture supernatants, serum and plasma.


      An IL-1 alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 alpha specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 alpha captured in plate.


      Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Biotinylated Human IL-1 alpha Antibody 1 x 60µl
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent M Concentrate 1 x 20ml
      20X Wash Buffer Concentrate 2 x 30ml
      Chromogen Substrate 1 x 8ml
      IL-1 alpha Microplate (12 x 8 well strips) 1 unit
      IL-1 alpha Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 12ml
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Other
      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Other
      • Immunology
      • Secreted Molecules
      • Other secreted molecules
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Interleukin ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
    • Relevance

      IL-1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
    • Cellular localization

      Secreted
    • Alternative names

      • Hematopoietin 1
      • IL 1
      • IL 1 alpha
      • Il 1a
      • IL1
      • IL1 ALPHA
      • IL1-a
      • IL1A
      • il1alpha
      • IL1F1
      • Ilia
      • Interleukin 1 alpha
      • Interleukin 1 alpha precursor
      • interleukin 1a
      • Interleukin1 alpha
      • OTTHUMP00000203692
      • OTTMUSP00000016430
      • Preinterleukin 1
      • Pro interleukin 1
      • Pro interleukin 1 alpha
      • Prointerleukin 1
      • RP23-160G19.8
      see all
    • Database links

      • Entrez Gene: 3552 Human
      • Omim: 147760 Human
      • SwissProt: P01583 Human

      Associated products

      • SimpleStep ELISA kits

        • Human IL-1a ELISA Kit (ab178008)

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab108864.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab108864? Please let us know so that we can cite the reference in this datasheet.

      ab108864 has not yet been referenced specifically in any publications.

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