• Product name

    Human IL-1 alpha ELISA Kit
    See all IL-1 alpha kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Sample A 22 459.8pg/ml 13 2.8%
    Sample B 21 72.6pg/ml 3.9 4.3%
    Sample n Mean SD CV%
    Sample A 8 259.9pg/ml 15 5.8%
    Sample B 8 58.6pg/ml 4.3 7.3%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    31.2 pg/ml - 1000 pg/ml
  • Recovery

    95.3 %

  • Assay time

    3h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-1a Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1a in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.

    A monoclonal antibody specific for IL-1a has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1a concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1a to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1a is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample. 

    This kit will recognize both endogenous and recombinant Human IL-1a.

    Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.


  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-1 alpha Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-1 alpha Control Silver 2 vials 4 vials
    IL-1 alpha Microplate (12 x 8 well strips) 1 unit 2 units
    IL-1 alpha Standard (Lyophilized) Yellow 2 vials 4 vials
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Relevance

    IL-1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
  • Cellular localization

  • Alternative names

    • Hematopoietin 1
    • IL 1
    • IL 1 alpha
    • Il 1a
    • IL1
    • IL1 ALPHA
    • IL1-a
    • IL1A
    • il1alpha
    • IL1F1
    • Ilia
    • Interleukin 1 alpha
    • Interleukin 1 alpha precursor
    • interleukin 1a
    • Interleukin1 alpha
    • OTTHUMP00000203692
    • OTTMUSP00000016430
    • Preinterleukin 1
    • Pro interleukin 1
    • Pro interleukin 1 alpha
    • Prointerleukin 1
    • RP23-160G19.8
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab46028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab46028



ab46028 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


This information is provided in the Protocol Booklet (pdf file) which can be found and down-loaded from the datasheet (top right corner). Please see section Assay Procedure 13.14:

"Read absorbance of each well on a spectrophotometer using 450 nm as the primary wavelength and optionally 620 nm (610 nm to 650 nm is acceptable) as the reference wavelength."

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