Human IL-1 alpha ELISA Set (without plates) (ab47350)

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Human IL-1 alpha ELISA Set (ab47350)

    Overview

    • Product name

      Human IL-1 alpha ELISA Set (without plates)

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 10 pg/ml

    • Range

      31.2 pg/ml - 1000 pg/ml

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s IL-1α Human in vitro ELISA Set is designed for the quantitative measurement of IL-1α in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


       


      This ELISA set is intended to measure human IL-1 alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


       


      The IL-1 alpha ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


       


      Recognizes both natural and recombinant human IL-1a.

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
      Detection biotinylated anti-IL-1 alpha antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
      IL-1 alpha Capture antibody 1 x 0.1ml 2 x 0.1ml 3 x 0.1ml
      IL-1 alpha Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials
      Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
      Streptavidin-HRP 1 vial 2 vials 3 vials

    • Research areas

    • Relevance

      IL-1 is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.

    • Alternative names

      • Hematopoietin 1
      • IL 1 alpha
      • IL1 alpha
      • IL1A
      • IL1F1
      • Interleukin 1 alpha
      see all

    • Database links

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab47350 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Sandwich ELISA
      Use at an assay dependent concentration.
      Notes
      Sandwich ELISA
      Use at an assay dependent concentration.

      Images

      • Human IL-1 alpha ELISA Set (ab47350)
        Human IL-1 alpha ELISA Set (ab47350)

      References (0)

      Publishing research using ab47350? Please let us know so that we can cite the reference in this datasheet.

      ab47350 has not yet been referenced specifically in any publications.

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