Human IL-1 alpha ELISA Set (without plates) (ab47350)
- Datasheet
- References
- Protocols
Overview
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Product nameHuman IL-1 alpha ELISA Set (without plates)
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Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 10 pg/ml
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Range31.2 pg/ml - 1000 pg/ml
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s IL-1α Human in vitro ELISA Set is designed for the quantitative measurement of IL-1α in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA set is intended to measure human IL-1 alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The IL-1 alpha ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL-1a.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformReagents
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-IL-1 alpha antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml IL-1 alpha Capture antibody 1 x 0.1ml 2 x 0.1ml 3 x 0.1ml IL-1 alpha Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
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RelevanceIL-1 is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
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Alternative names
- Hematopoietin 1
- IL 1 alpha
- IL1 alpha
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Database links
- Entrez Gene: 3552 Human
- SwissProt: P01583 Human
Applications
Our Abpromise guarantee covers the use of ab47350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Human IL-1 alpha ELISA Set (ab47350)
Protocols
References
ab47350 has not yet been referenced specifically in any publications.
