Overview

  • Product name
    Human IL-1 beta ELISA Set (without plates)
    See all IL-1 beta kits
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    15.6 pg/ml - 500 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-1β Human in vitro ELISA Set is designed for the quantitative measurement of IL-1β in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


     


    This ELISA Set is intended to measure human IL-1b in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


     


    The IL-1b ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


     


    Recognizes both natural and recombinant human IL-1b.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-1 beta antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-1 beta Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    IL1 beta standard Yellow 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • Research areas
  • Function
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • Tissue specificity
    Expressed in activated monocytes/macrophages (at protein level).
  • Sequence similarities
    Belongs to the IL-1 family.
  • Post-translational
    modifications
    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • Cellular localization
    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • IL 1
    • IL 1 beta
    • IL-1 beta
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin-1 beta
    • OAF
    • OTTHUMP00000162031
    • Preinterleukin 1 beta
    • Pro interleukin 1 beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IL-1 beta Standard curve ranging from 15.6 to 500 pg/ml

References

ab47351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for contacting Abcam.



The antibodies of ab47351 are both monoclonals, raised in mice.



If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting us.

1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step. Unfortunately, ELISA plates are not supplied.

2) n/a. If you require kits including plates I may recommend ab108865 and ab108867, respectively.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=108865).
Click here (or use the following: https://www.abcam.com/index.html?datasheet=108867).

3) We can recommend any polystyrene 1x96 well or 12x8 well plate suitable for ELISA.

4) Please find attached the protocols for ab48478 and ab47351. The suggested procedures may need some optimization depending on the plastic, matrix, standard range.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry.

For ab47351 you can use 96 well ELISA plates. I can recommend to purchase ELISA plates specifically. to ensure they are of the higher quality required. I am sorry wedo not sell these from Abcam, I can recommendto do an internet search to find some.

ab48487 is a CRLF2 antibody that I am sorry is no longer available. Could you confirm the product code?

I hope this will be helpful, and I look forward to hearing from you with regards to the second item.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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