Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.65 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IL-1 R4 ELISA Kit (ST2)
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 2 pg/ml
Range1.65 pg/ml - 1200 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.43 91% - 105% Serum 109.6 98% - 117% Plasma 84.06 67% - 102%
Assay time5h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human IL-1 R4 ELISA Kit (ST2) (ab100563) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 R4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-1 R4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 R4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 R4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-1 R4 2 vials IL-1 R4 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-1 R4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.
- FIT 1
- Growth stimulation expressed
- SwissProt: Q01638-2 Human
ab100563 has been referenced in 2 publications.
- Hsieh CC et al. Dynamic changes of soluble ST2 levels predicted fatality and were involved in coagulopathy in dengue fever in the elderly. PLoS Negl Trop Dis 13:e0007974 (2019). PubMed: 31877138
- Kotsiou OS et al. Pleural Effusion IL-33/sST2 Levels and Effects of Low and High IL-33/sST2 Levels on Human Mesothelial Cell Adhesion and Migration. Inflammation 42:2072-2085 (2019). PubMed: 31429014