Overview

  • Product name

    Human IL-10 ELISA Kit
    See all IL-10 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1 pg/ml
  • Range

    2.34 pg/ml - 150 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.37 87% - 110%
    Serum 101.94 87% - 108%
    Plasma 102.39 93% - 109%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range.


     

  • Notes

    Optimization may be required with urine samples

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IL-10 measured in culture supernatants from control (-) or activated cell lines (dilution range 1/1-1/00), stimulated for 24 hours with 50 ng x mL-1 of PMA (P)(ab120297) and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants (dilution range 1/10-1/1000), from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).

  • Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).

  • Standard curve (assay diluent A) with background signal subtracted (duplicates; +/- SD).

  • Dilution curves (in assay diluent B) of human IL-10 (ab100549) and NIBSC standard (93/722). One ng of standard IL-10 corresponds to 0.94 (+/- 0.08) U NIBSC standard. Background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100549

  • Representative standard curve using ab100549

Protocols

References

This product has been referenced in:

  • Cheng H  et al. KrasG12D mutation contributes to regulatory T cell conversion through activation of the MEK/ERK pathway in pancreatic cancer. Cancer Lett 446:103-111 (2019). Read more (PubMed: 30664964) »
  • Dong Z  et al. EGFR may participate in immune evasion through regulation of B7-H5 expression in non-small cell lung carcinoma. Mol Med Rep 18:3769-3779 (2018). Read more (PubMed: 30106102) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100566 IL2 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-2 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100570 IL4 Human ELISA Kit
o Capture Antibody
§ Mouse, monoclonal
§ Not tested for cross-reactivity with mouse samples.
o Detection Antibody
§ Goat, polyclonal
§ Detects the region His25­Ser153 (Accession # NP_000580)
§ Not tested for cross-reactivity with mouse samples.
· ab100689 Interferon gamma Mouse ELISA Kit
o Capture Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
· ab100697 IL10 Mouse ELISA Kit
o Capture Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
· ab100706 IL2 Mouse ELISA Kit
o The detection antibody did not show any cross-reactivity with Human IL-2 from testing. Thus, this kit is not expected to cross react with human samples.
· ab100710 IL4 Mouse ELISA Kit
o The capture antibody did not show any cross-reactivity with Human IL-4 from testing. Thus, this kit is not expected to cross react with human samples.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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