Overview

  • Product name

    Human IL-10 ELISA Kit
    See all IL-10 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    > 0.12 ng/ml
  • Range

    0.125 ng/ml - 8 ng/ml
  • Recovery

    98 %

  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-10 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 levels in cell culture supernatants, serum, plasma and tissue extracts.


    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate. This assay recognizes both natural and recombinant Human IL-10.


    Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve Using ab108871.

Protocols

References

ab108871 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The equivalent IL10 assay, with 12 strips of 8 wells each, is ab100549. Product ab108871 comes with a single plate, not strips, but does use an HRP-conjugated streptavidin, as does the TNF alpha ELISA, ab100654.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=100549).

Please contact us if you have any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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