Overview

  • Product name
    Human IL-10 ELISA Kit High Sensitivity
    See all IL-10 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3 7.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 10.2%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1.3 pg/ml
  • Range
    1.56 pg/ml - 50 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94 % - %
    Serum 80 59% - 93%

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-10 Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in Human sera, plasmas, buffered solutions and cell culture media.


    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-10.


    Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range.


     


     


     

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab46059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab46059

Protocols

References

This product has been referenced in:
  • Sahbudak Bal Z  et al. Diagnostic Accuracy of Interleukin-6, Interleukin-8, and Interleukin-10 for Predicting Bacteremia in Children with Febrile Neutropenia. Turk J Haematol 34:254-257 (2017). Read more (PubMed: 28148470) »
  • Sammour I  et al. The Effect of Gender on Mesenchymal Stem Cell (MSC) Efficacy in Neonatal Hyperoxia-Induced Lung Injury. PLoS One 11:e0164269 (2016). Read more (PubMed: 27711256) »
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for contacting us.

I am sorry about the continued problems that you have been experiencing using this kit. Unfortunately I do not have any further suggestions to help with this issue. I have created a credit note/refund for the original purchase. Your credit note ID is 20001.

I am sorry that this product did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

I would like to suggest that you try using another kit to determine whether this is a product specific issue. If you provide me with the orginal Abcam order confirmation code I can send you a free of charge replacement for this product.



Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

I've passed this information on to the lab for you.

They feel that the data enclosed are pretty good, except the blank which is indeed a little bit high, and which should rather be considered as a zero. They also also would like to confirm what you use as Blank/zero?

Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for that additional information. The matrix effect could be called quenching. The high protein concentration of serum samples or components contained in samples can affect assay response to the analyte (IL-10) and give background. If you dilute samples, then the matrix is altered and the recovery of analyte is better. The results obtained should be multiplied by the dilution factor.There is two different diluents provided in the kit, depending of what kind of samples are assaying (serum/plasmas or cell supernatents). We do recommend using these. I hope this information is helpful to you. Please do not hesitate to contact us should you have any questions.

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Answer

Thank you for contacting Abcam.

I have had the opportunity to speak with the lab regarding this issue. The low signal obtained may be linked to the use of human serum as standard curve diluent. Have you diluted the standards in human serum or in standard diluent buffer PBS/BSA? They also advise diluting the samples 1:2 to minimize the matrix effect.

Please let me know if you have any questions.

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Question
Answer

Thank you for your call today, and for letting us know about the trouble with this kit. As we discussed, please forward any data you have using this kit, and I will see if there are any suggestions that we can make to improve the results. I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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Question
Answer

Thank you for your call yesterday and for your patience while i have been in touch with the lab regarding these IL10 kits. Here is the reply that I received from my contact at the lab: "To be on the safe side regarding sensitivity, my opinion would be to use the High Sensitivity format, with dilution factor for plasmas 1:2 or 1:5. On the other hand, for someone new to ELISA, the Non-High Sensitivity one is easier to assay (less steps & more robust). According to general knowledge, IL-10 concentration is increased in HIV positive plasmas, so the detection level should be convenient." I hope this information will be useful in determining which of these kits will be most suitable for your studies, but please let me know if you have any further questions or if there is anything else that we can do for you.     v\:* {behavior:url(#default#VML);} o\:* {behavior:url(#default#VML);} w\:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);}  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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