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The standards were dissolved in the provided diluent. What is the matrix effect? So despite the fact that we are getting low signal, do you suggestion diluting samples 1:2? I appreciate your help. Thank you very much for your continued help on this kit.
Asked on Feb 06 2012
Thank you for that additional information. The matrix effect could be called quenching. The high protein concentration of serum samples or components contained in samples can affect assay response to the analyte (IL-10) and give background. If you dilute samples, then the matrix is altered and the recovery of analyte is better. The results obtained should be multiplied by the dilution factor.There is two different diluents provided in the kit, depending of what kind of samples are assaying (serum/plasmas or cell supernatents). We do recommend using these. I hope this information is helpful to you. Please do not hesitate to contact us should you have any questions.
Answered on Feb 06 2012