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We have rerun some samples and standards using all the suggestions you provided on the phone and via email. We ran only two test strips and the results still look odd. Attached is the data from those two strips.. I probably need to explain what each are. The spiked sample are way off. When I spiked a sample I mixed put 100ul sample with 90ul of a particular standard (all I had left of them) and the pipetted 100ul of this mixture to the plate just like the regular sample and standards. We ran, a blank, low, mid and high standard twice (SA, SC, etc.) and (PA, PB, etc.) and these are highlighted in the excel sheet. The others are either serum from control subjects or as indicated with the microliters, the subject samples spiked with standards, for which the results don't indicate the expected numbers. As you can also see, the absorbance on the zero standard (the blanks) is high and the range of absorbances is small and low. Can you please provide some help? We appreciate any tips you can provide. We have corresponded many times via phone and through email with you and are looking forward to getting some useful tips.
Asked on Feb 28 2012
I've passed this information on to the lab for you.
They feel that the data enclosed are pretty good, except the blank which is indeed a little bit high, and which should rather be considered as a zero. They also also would like to confirm what you use as Blank/zero?
Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on Feb 28 2012