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Thank you for passing this on. We are eagerly and anxiously awaiting for your reply and perhaps some tips. The blank we use, is included in the standard curve as a "zero." Even though the absorbance is high. We are concerned because our some of our unknown samples give less than the "zero" because the blank/zero is at a higher absorbance. Furthermore, we are not seeing recovery of the spiked amounts in our samples. Please let me know if there is any other information we can provide. We appreciate your help and are looking forward to getting this working.
Asked on Feb 28 2012
I would like to suggest that you try using another kit to determine whether this is a product specific issue. If you provide me with the orginal Abcam order confirmation code I can send you a free of charge replacement for this product.
Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on Feb 28 2012