Product nameHuman IL-10 ELISPOT Kit (Reagents for 10 x 96 Well Plates)
See all IL-10 kits
Assay typeSandwich (quantitative)
Species reactivityReacts with: Human
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
The Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Recognizes natural human IL-10
Tested applicationsSuitable for: ELISpotmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 x 96 tests Biotinylated detection antibody, clone B-T10 (Lyophilized) 2 vials Human IL-10 Capture antibody 2 x 0.5ml
FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Sequence similaritiesBelongs to the IL-10 family.
- Information by UniProt
- Cytokine synthesis inhibitory factor
Our Abpromise guarantee covers the use of ab46601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab46601 has not yet been referenced specifically in any publications.