• Product name
    Human IL-10RB ELISA Kit
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 129.1 120% - 140%
    Serum 121.9 110% - 129%
    Plasma 104.5 92% - 112%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-10RB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10RB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-10RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-10RB 2 vials
    IL-10RB Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-10RB Standard (lyophilized) 2 x 1ml
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29.
  • Involvement in disease
    Defects in IL10RB are the cause of inflammatory bowel disease type 25 (IBD25) [MIM:612567]. It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • Sequence similarities
    Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • CDw210b
    • CRF2 4
    • CRF2-4
    • CRFB4
    • Cytokine receptor class II member 4
    • Cytokine receptor class-II member 4
    • Cytokine receptor family 2 member 4
    • D21S58
    • D21S66
    • I10R2_HUMAN
    • IL-10 receptor subunit beta
    • IL-10R subunit 2
    • IL-10R subunit beta
    • IL-10R2
    • IL-10RB
    • IL10 receptor subunit beta
    • IL10R subunit 2
    • IL10R subunit beta
    • IL10R2
    • IL10RB
    • Interleukin 10 receptor subunit 2
    • Interleukin 10 receptor subunit beta
    • Interleukin-10 receptor subunit 2
    • Interleukin-10 receptor subunit beta
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100550

  • Representative standard curve using ab100550



ab100550 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab100550.
Please use the links above to contact us or submit feedback about this product.


Sign up