Overview

  • Product name

    Human IL-12 p70 ELISA Kit
    See all IL12 p70 kits
  • Detection method

    Colorimetric
  • Precision

    Inter-assay
    Sample n Mean SD CV%
    1 24 67pg/ml 3.89 5.8%
    2 24 158pg/ml 9.48 6%
    3 24 322pg/ml 23.2 7.2%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    7.8 pg/ml - 500 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human IL-12(p70) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213791) is designed for the quantitative detection of Human IL-12(p70) in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-12(p70) has been pre-coated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: (I23-S328)+(R23-S219)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(p70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-12(p70) amount of sample captured in plate. The standard product used in this kit is recombinant human IL-12(p70) with the molecular mass of 75 kDa.

  • Notes

    Interleukin (IL)-12 is a 70 kDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. The IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT-18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12(p70).

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IL-12(p70) ELISA Kit (ab213791) Standard Curve

Protocols

References

ab213791 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213791.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up