Overview

  • Product name

    Human IL-12 p70 ELISA Kit
    See all IL12 p70 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.2%
  • Sample type

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    13.6 pg/ml
  • Range

    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 93% - 101%
    Heparin Plasma 85 80% - 89%
    EDTA Plasma 99 97% - 101%
    Citrate Plasma 93 92% - 94%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IL-12 p70 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 protein in serum, plasma and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits, IL-12A (p35) and IL-12B (p40).  This kit recognizes specifically the IL-12 p70 total heterodimer protein.  IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFNγ.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab223592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-12 p70 were measured in duplicates, interpolated from the IL-12 p70 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 2.5%, plasma (citrate) 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of IL-12 p70 were measured.

Protocols

References

ab223592 has not yet been referenced specifically in any publications.

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