Overview

  • Product name

    Human IL-12 p70 ELISA Kit
    See all IL-12 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6 8.9%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 2.2 pg/ml
  • Range

    6.25 pg/ml - 200 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Serum 91 86% - 94%

  • Assay time

    3h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-12 p70 (Interleukin- 12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 in supernatants, buffered solutions or serum and plasma samples.

    A monoclonal antibody specific IL-12 p70 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 p70 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 p70 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 p70 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-12 p70.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab46143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  •  

    Representative Standard Curve using ab46143

Protocols

References

ab46143 has not yet been referenced specifically in any publications.

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