Human IL-12 p70 ELISA Kit (ab100552)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.8 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-12 p70 ELISA Kit
See all IL-12 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
0.8 pg/ml - 600 pg/ml -
Recovery
88 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.52 88% - 109% Plasma 85.63 79% - 101% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-12 p70 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human IL-12 p70 in normal serum with ab100552, only in serum spiked with Human IL-12 p70.
Get results in 90 minutes with Human IL-12p40/p70 ELISA Kit (ab178009) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples.
The standard is affinity purified.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-12 p70 2 vials IL-12 p70 Microplate (12 x 8 wells) 1 unit Recombinant IL-12 p70 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CLMF
- CLMF p35
- CLMF p40
see all -
Database links
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- Omim: 161560 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100552 has not yet been referenced specifically in any publications.