Overview

  • Product name

    Human IL-13 ELISA Kit
    See all IL-13 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.15 pg/ml
  • Range

    0.16 pg/ml - 40 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.65 88% - 107%
    Serum 96.35 84% - 103%
    Plasma 94.39 82% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-13 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human IL-13 ELISA Kit (ab178014) from our SimpleStep ELISA® range.


     

  • Notes

    Optimization may be required with urine samples

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100553

  • Representative standard curve using ab100553

Protocols

References

This product has been referenced in:

  • Yamamoto M  et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). Read more (PubMed: 29385874) »
  • Hosseinkhani B  et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). Read more (PubMed: 30131806) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for your calls this week and I appreciate your patience. Unfortunately, all of the components of our kits are not available for individual purchase, which includes the detection antibody of ab100533. We can only provide this component with the full size kit. We apologize for any inconvenience this may cause. Please let us know if you have any questions or if there is anything else that we might be able to do for you.

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Answer

Thanks for your email. Sorry for the delay. I am looking into the possibility of providing this antibody to you separately from ab100553. Currently it is not sold separately and is only sold as part of the IL13 Human ELISA Kit you purchased. I will follow up with you as soon as I find out whether or not we can provide this antibody to you. Thanks for your patience.

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Answer

Thank you for your email.  I have addressed your questions below.   1) The protocol mentions that the biotinilated IL13 detection antibody can be stored for only 5 days at 4 degrees C once resuspended with 100ul buffer. This sounds like a short time be able to store an antibody. Is it possible this antibody can be stored for longer? While 5 days at 4ºC is a conservative estimate, we would still not recommend storing the resuspended antibody for a longer period of time and especially not freezing it as freeze-thaw cycles can degrade the antibody.  As you may know, the more dilute an antibody/protein solution is, the lower concentration of stabilizers presents and therefore the less stable it is.  Please note that 2 vials of detection antibody are supplied in each kit (1 vial is enough for half a microplate) to limit storage issues. 2) Some other questions about reagent storage. The protocol does not give details regarding storage of the HRP-SA reagent, the TMB or the stop solution. Storage information can be found on page 3 of the manual.  As you can see, the whole kit can be stored at 2ºC to 8ºC for up to 6 months or -20ºC for long-term storage.   Once opened/thawed the microplate and other reagents including the HRP-streptavidin (as 6000X concentrate), TMB and stop solution must be used within 1 month at 2º to 8ºC. I hope this is helpful. Please contact us again if you have any further questions.

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