Overview

  • Product name

    Human IL-13 ELISA Kit
    See all IL-13 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall 4 3.3%
    Inter-assay
    Sample n Mean SD CV%
    overall 3 2.5%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    39 pg/ml
  • Range

    78 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 67 65% - 69%
    Cell culture media 86 82% - 91%
    Citrate Plasma 59 57% - 61%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IL-13 (Interleukin-13) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-13 protein in human cell culture supernatant and Human serum and plasma samples.


    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab178014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IL-13 was measured in undiluted, 2-fold, 4-fold and 8-fold diluted cell culture supernatants of the PHA stimulated PBMC, unstimulated PBMC and media. Raw data values (mean +/-SD, n=2) are graphed. The dotted line represents zero sample background.

  • The concentrations of IL-13 were interpolated from data values shown in Figure 2 using IL-13 standard curve and corrected for sample dilution. The mean IL‑13 concentration was determined to be 2,954 pg/mL in stimulated PBMC supernatants and 73 pg/mL in unstimulated PBMC.

Protocols

References

This product has been referenced in:

  • Furukawa S  et al. Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. Sci Rep 7:42413 (2017). ELISA ; Human . Read more (PubMed: 28205524) »
  • Séguier S  et al. Inhibition of the differentiation of monocyte-derived dendritic cells by human gingival fibroblasts. PLoS One 8:e70937 (2013). Read more (PubMed: 23936476) »
See all 2 Publications for this product

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