Human IL-13 ELISA Kit (ab288591)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.662 pg/ml
- Range: 4.688 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-13 ELISA Kit
See all IL-13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 4.1% Inter-assay Sample n Mean SD CV% Supernatant 3 4.91% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.662 pg/ml -
Range
4.688 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 86% - 104% Serum 89 88% - 90% Hep Plasma 94 91% - 97% EDTA Plasma 95 91% - 101% Cit plasma 86 82% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-13 ELISA Kit (ab288591) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in serum, EDTA plasma, Hep plasma, Cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-13 with 1.662 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-13 Capture Antibody 1 x 600µl 10X Human IL-13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-13 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Allergic rhinitis
- ALRH
- BHR 1
see all -
Database links
- Entrez Gene: 3596 Human
- Omim: 147683 Human
- SwissProt: P35225 Human
- Unigene: 845 Human
Images
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Example of human IL-13 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentration of native IL-13 was measured in duplicate at different sample concentrations. Undiluted samples are 1:20 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Standard curve comparison between the original Human IL-13 SimpleStep ELISA (ab178014) and current Human IL-13 SimpleStep ELISA (ab288591).
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Standard curve comparison between Human IL-13 SimpleStep ELISA kit (ab288591) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab288591 has not yet been referenced specifically in any publications.