• Product name

    Human IL-13 ELISA Kit
    See all IL-13 kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Sample A 8 99.04pg/ml 0.476 0.48%
    Sample B 8 13.47pg/ml 0.423 3.14%
    Sample n Mean SD CV%
    Sample A 58 99.95pg/ml 1.439 1.43%
    Sample B 58 13.27pg/ml 0.767 5.77%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1.5 pg/ml
  • Range

    3.12 pg/ml - 100 pg/ml
  • Recovery

    102.3 %

  • Assay time

    2h 10m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-13 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-13 in Human serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for IL-13 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-13.

    Get results in 90 minutes with Human IL-13 ELISA Kit (ab178014) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-13 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-13 Control Silver 2 vials 4 vials
    IL-13 Microplate (12 x 8 well strips) 1 unit 2 units
    IL-13 standard Yellow 2 vials 4 vials
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Function

    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease

    Allergic rhinitis
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

  • Information by UniProt
  • Alternative names

    • Allergic rhinitis
    • ALRH
    • BHR 1
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • interleukin13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC 30
    • NC30
    • P 600
    • P600
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab46038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab46038



ab46038 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


I received confirmation from the laboratory that samples/standards are co-incubate with the biotinylated Antibody (1h30 room temperature) in the IL-13 ELISA Kit. There's then a washing step and the incubation of the streptavidin-HRP as detailed in the protocol. You therefore do not need an incubation step of the samples/standards alone prior to adding the antibody. I hope this clarification helps, please do not hesitate to contact me if I can be of further assistance,

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