• Product name

    Human IL-13 ELISA Set (without plates)
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1.5 pg/ml
  • Range

    3.21 pg/ml - 100 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-13 Human in vitro ELISA Set is designed for the quantitative measurement of IL-13 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


    This ELISA Set is intended to measure human IL-13 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    The IL-13 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


    Recognizes both natural and recombinant human IL-13.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-13 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-13 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    IL-13 standard Yellow 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • Research areas

  • Relevance

    Human IL-13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL-13 (P600) cDNA as a probe. Human IL-13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL-13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF. IL-13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL-13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL-13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58% amino acid sequence identity. Although human and mouse IL-13 are equally active on human cells, human IL-13 is much less active than mouse IL-13 on mouse cells. Human IL-13 and human IL-4 also share approximately 30% sequence homology and have similar biological functions.
  • Cellular localization

  • Alternative names

    • ALRH
    • BHR1
    • IL13
    • Interleukin 13
    • P600
    see all
  • Database links


Our Abpromise guarantee covers the use of ab47353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • IL-13 Standard curve ranging from 3.12 to 100 pg/mL


ab47353 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your call last week and for your patience while I have been in touch with the lab about ab47353. Both the capture and detection antibodies are produced in mouse. I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.  

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