Human IL-13 ELISpot Kit (ab281964)

Human IL-13 ELISpot Kit (ab281964) is a highly specific immunoassay for the quantitative determination of human IL-13 production and secretion from T-cells at a single cell level in conditions, closely comparable to the in-vivo environment with minimal cell manipulation.

A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

For research use only - not intended for diagnostic use.

Please note for 001PC (1 x 96 tests) detection antibody is provided in liquid form, for 005PC (5 x 96 tests) it is provided lyophilized.

• Immunology
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