Overview

  • Product name

    Human IL-13R alpha 2 ELISA Kit
    See all IL-13 receptor alpha 2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich
  • Sensitivity

    0.4 ng/ml
  • Range

    0.4 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 77.79 71% - 85%
    Plasma 74.7 67% - 81%
    Cell culture media 81.45 72% - 89%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's IL-13 Receptor alpha 2 Human ELISA Kit (ab193704) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-13 Receptor alpha 2 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for human IL-13 Receptor alpha 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-13 Receptor alpha 2 present in the samples. The wells are washed and biotinylated anti-human IL-13 Receptor alpha 2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-13 Receptor alpha 2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Detection Antibody IL-13 Receptor alpha 2 (Biotinylated anti-Human IL-13 Receptor alpha 2) 2 vials
    Human IL-13R alpha 2 Standards (lyophilized) 2 vials
    Pre-coated Human IL-13R alpha 2 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 5 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Cancer/testis antigen 19
    • CD213a2
    • CT19
    • I13R2_HUMAN
    • IL-13 receptor subunit alpha-2
    • IL-13R subunit alpha-2
    • IL-13R-alpha-2
    • IL-13RA2
    • IL13 receptor
    • IL13BP
    • IL13R
    • Il13ra2
    • Interleukin 13 binding protein
    • Interleukin 13 receptor alpha 2 chain
    • Interleukin-13 receptor subunit alpha-2
    • Interleukin-13-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab193704 has not yet been referenced specifically in any publications.

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