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  1. Link

    human-il-17-a-elispot-antibody-pair-ab281969.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Human IL-17 A ELISpot Antibody Pair (ab281969)

  • Datasheet
  • Protocol Booklet
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Key features and details

  • Assay type: ELISPOT
  • Detection method: Colorimetric
  • Sample type: Suspension cells
  • Reacts with: Human

Overview

  • Product name

    Human IL-17 A ELISpot Antibody Pair
    See all IL-17A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 12 372cells/well 11%
    Overall 12 439cells/well 7%
    Overall 12 299cells/well 4%
    Overall 12 157cells/well 10%
    Overall 12 69cells/well 15%
    Overall 12 31cells/well 21%
  • Sample type

    Suspension cells
  • Assay type

    ELISPOT
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-17A ELISpot Antibody Pair (ab281969) is an antibody pair designed for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level by the ELISpot method.


    For the quantitative determination of the frequency of Human IL-17A-producing cells.


    For research use only - not intended for diagnostic use.

  • Tested applications

    Suitable for: ELISpotmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Biotinylated Human IL-17 A detection antibody 2 vials
    Human IL-17 A Capture Antibody 2 x 0.5ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Processes
  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q16552 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all
  • Database links

    • Entrez Gene: 3605 Human
    • Omim: 603149 Human
    • SwissProt: Q16552 Human
    • Unigene: 41724 Human

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab281969 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISpot
    Use at an assay dependent concentration.
    Notes
    ELISpot
    Use at an assay dependent concentration.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab281969? Please let us know so that we can cite the reference in this datasheet.

    ab281969 has not yet been referenced specifically in any publications.

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