Overview

  • Product name

    Human IL-17 ELISA Kit
    See all IL-17 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 7.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.1%
  • Sample type

    Serum, Plasma, Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.5 pg/ml
  • Range

    1.6 pg/ml - 100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 113.6 % - %
    Serum 107 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-17 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-17 concentrations in Cell culture supernatant, serum and plasma (citrate, heparin).


    Human IL-17 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-17 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-17 captured on the plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-Human IL-17 monoclonal antibody 1 x 70µl
    Control high 1 vial
    Control low 1 vial
    Human IL-17 Standard Lyophilized (200 pg/mL on reconstitution) 2 vials
    Microplate coated with monoclonal antibody to Human Il-17 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119535.

Protocols

References

This product has been referenced in:

  • Wang CY  et al. Effect of Liuweibuqi capsules on CD4+CD25+Foxp3+ regulatory T cells, helper T cells and lung function in patients with stable chronic obstructive pulmonary disease complicated with lung Qi deficiency. J Thorac Dis 10:2700-2711 (2018). Read more (PubMed: 29997932) »
  • Iki H  et al. Effect of exercise therapy on cytokine secretion in the saliva of bedridden patients. J Phys Ther Sci 28:2871-2876 (2016). Human . Read more (PubMed: 27821953) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas específicos para ratón, y específicos para humano. Después de estudiar la homología entre secuencias, me temo que no podemos predecir que exista reactividad cruzada entre especies. Experimentalmente tampoco tenemos datos para confirmarlo.

Siento no poder ser de mas ayuda. No dudes en contactarnos para cualquier otra consulta.

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