Overview

  • Product name

    Human IL-17A ELISA Kit
    See all IL-17A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 10.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 6.8%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.1 pg/ml
  • Range

    1.56 pg/ml - 100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97 97% - 98%
    Cell culture media 92 82% - 108%
    Heparin Plasma 96 86% - 115%
    EDTA Plasma 106 104% - 110%
    Citrate Plasma 101 94% - 108%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    Human IL-17A SimpleStep ELISA kit (ab176111) has been re-developed with new capture and detectoe antibodies.This new kit has the same name but a different product number (ab216167). We have identified new recombinant monoclonal antibodies to use in the SimpleStep ELISA platform that provide a higher sensitivity when quantifying IL-17A in human serum, plasma and cell culture supernatants.


     IL-17A in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A protein in human serum, plasma, and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    IL-17A is a proinflammatory cytokine that is secreted by activated T cells. It is a disulfide-linked homodimer with both glycosylated and nonglycosylated forms. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-17A Capture Antibody 1 x 600µl
    10X Human IL-17A Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human IL-17A Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab216167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 13-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 10%, and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC supernatant and 43.6 pg/mL in neat unstimulated PBMC supernatant.

  • The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of IL-17A were measured in supernatant samples in 5 different dilutions in duplicates and interpolated from the IL-17 A standard curve. The interpolated values are plotted (mean +/- SD, n=5). The mean IL-17 A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 43.6 pg/mL in neat unstimulated supernatants and undetectable in neat media (not shown).

Protocols

References

ab216167 has not yet been referenced specifically in any publications.

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