For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    human-il-17a-elisa-kit-ab216167.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Cytokines Interleukins
Share by email
RecombinantSimpleStep

Human IL-17A ELISA Kit (ab216167)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Other - Human IL-17A ELISA Kit (ab216167)
  • Human IL-17A standard curve comparison data.
  • Example of human IL-17A standard curve.
  • Interpolated concentrations of native IL-17A in PHA-M stimulated and unstimulated human PBMC cell culture supernatant samples.
  • Interpolated concentrations of spike IL-17A in human serum and plasma samples.
  • Comparison of IL-17A in unstimulated and PHA-M stimulated human PBMC cell supernatants.
  • Sandwich ELISA - Human IL-17A ELISA Kit (ab216167)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.1 pg/ml
  • Range: 1.56 pg/ml - 100 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

ELISA
Product image
Human IFN gamma High Sensitivity ELISA Kit (ab236895)
Pair
Product image
Human IL-1beta Antibody Pair - BSA and Azide free (ab241807)
Primary
Anti-5HT Receptor antibody (ab56004)

View more associated products

Overview

  • Product name

    Human IL-17A ELISA Kit
    See all IL-17A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3 10.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5 6.8%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.1 pg/ml
  • Range

    1.56 pg/ml - 100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97 97% - 98%
    Cell culture media 92 82% - 108%
    Hep Plasma 96 86% - 115%
    EDTA Plasma 106 104% - 110%
    Cit plasma 101 94% - 108%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-17A ELISA Kit (ab216167) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17A protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17A with 1.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-17A is a proinflammatory cytokine that is secreted by activated T cells. It is a disulfide-linked homodimer with both glycosylated and nonglycosylated forms. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-17A Capture Antibody 1 x 600µl
    10X Human IL-17A Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human IL-17A Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Processes
  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q16552 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8)
    • interleukin 17A
    • Interleukin-17A
    • interleukin17
    • Interleukin17A
    • OTTHUMP00000016597
    • OTTMUSP00000046003
    see all
  • Database links

    • Entrez Gene: 3605 Human
    • Omim: 603149 Human
    • SwissProt: Q16552 Human
    • Unigene: 41724 Human

    Associated products

    • Alternative Versions

      • Human IL-17A Antibody Pair - BSA and Azide free (ab241882)
    • ELISA kits

      • Human IL-17A Matched Antibody Pair Kit (ab218807)

    Images

    • Other - Human IL-17A ELISA Kit (ab216167)
      Other - Human IL-17A ELISA Kit (ab216167)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human IL-17A standard curve comparison data.
      Human IL-17A standard curve comparison data.

      Standard curve comparison between human IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 13-fold increase in sensitivity.

    • Example of human IL-17A standard curve.
      Example of human IL-17A standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native IL-17A in PHA-M stimulated and unstimulated human PBMC cell culture supernatant samples.
      Interpolated concentrations of native IL-17A in PHA-M stimulated and unstimulated human PBMC cell culture supernatant samples.

      The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 10%, and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC supernatant and 43.6 pg/mL in neat unstimulated PBMC supernatant.

    • Interpolated concentrations of spike IL-17A in human serum and plasma samples.
      Interpolated concentrations of spike IL-17A in human serum and plasma samples.

      The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Comparison of IL-17A in unstimulated and PHA-M stimulated human PBMC cell supernatants.
      Comparison of IL-17A in unstimulated and PHA-M stimulated human PBMC cell supernatants.

      Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of IL-17A were measured in supernatant samples in 5 different dilutions in duplicates and interpolated from the IL-17 A standard curve. The interpolated values are plotted (mean +/- SD, n=5). The mean IL-17 A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 43.6 pg/mL in neat unstimulated supernatants and undetectable in neat media (not shown).

    • Sandwich ELISA - Human IL-17A ELISA Kit (ab216167)
      Sandwich ELISA - Human IL-17A ELISA Kit (ab216167)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab216167? Please let us know so that we can cite the reference in this datasheet.

    ab216167 has been referenced in 2 publications.

    • Tian X  et al. RNA-Seq Identifies Marked Th17 Cell Activation and Altered CFTR Expression in Different Atopic Dermatitis Subtypes in Chinese Han Populations. Front Immunol 12:628512 (2021). PubMed: 33868246
    • Yang X  et al. IL-17a exacerbates hepatic ischemia-reperfusion injury in fatty liver by promoting neutrophil infiltration and mitochondria-driven apoptosis. J Leukoc Biol 108:1603-1613 (2020). PubMed: 32531822

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab216167.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.