Overview

  • Product name

    Human IL-17a ELISA Kit
    See all IL-17a kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 6 3.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6 5.2%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 3 pg/ml
  • Range

    3.12 pg/ml - 100 pg/ml
  • Recovery

    105 %

    Sample specific recovery
    Sample type Average % Range
    Serum 121 113% - 130%

  • Assay time

    3h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-17a Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative of IL-17a in supernatants, buffered solutions, serum, plasma and other body fluids.


    A monoclonal antibody specific for IL-17a has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-17a concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-17a are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-17a present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-17a.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab83688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IL-17 measured in cell culture supernatants (dilution range 1/1-1/100) from control (PBMC) or treated ((PBMC+PHA) 48 hours; 2% PHA-M; LifeTechnologies) cells (duplicates +/- SD).

  • Representative Standard Curve using ab83688

Protocols

References

This product has been referenced in:

  • Caselli G  et al. Pharmacological characterisation of CR6086, a potent prostaglandin E2receptor 4 antagonist, as a new potential disease-modifying anti-rheumatic drug. Arthritis Res Ther 20:39 (2018). Read more (PubMed: 29490676) »
  • Huang G  et al. Platelet-rich plasma shows beneficial effects for patients with knee osteoarthritis by suppressing inflammatory factors. Exp Ther Med 15:3096-3102 (2018). Read more (PubMed: 29599843) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas específicos para ratón, y específicos para humano. Después de estudiar la homología entre secuencias, me temo que no podemos predecir que exista reactividad cruzada entre especies. Experimentalmente tampoco tenemos datos para confirmarlo.

Siento no poder ser de mas ayuda. No dudes en contactarnos para cualquier otra consulta.

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