Overview

  • Product name

    Human IL-17A Receptor ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    12.35 pg/ml - 3000 pg/ml
  • Recovery

    81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 81.54 70% - 92%
    Serum 87.64 83% - 104%
    Plasma 73.27 67% - 87%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-17A Receptor Human ELISA (Enzyme Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17A in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human IL-17A 2 vials
    IL-17A Microplate (12 x 8 wells) 1 x 200µl
    Recombinant Human IL-17A Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling.
  • Tissue specificity

    Widely expressed.
  • Involvement in disease

    Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5) [MIM:613953]. CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • Sequence similarities

    Contains 1 SEFIR domain.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • CD217
    • CDw217
    • hIL 17R
    • I17RA_HUMAN
    • IL 17 receptor A
    • IL 17RA
    • IL-17 receptor A
    • IL-17RA
    • IL17A Receptor
    • IL17R
    • IL17RA
    • Interleukin 17 receptor A
    • Interleukin-17 receptor A
    • MGC10262
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100558

  • Representative standard curve using ab100558

Protocols

References

ab100558 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and interest in our product. I can confirm that this kit (Ref: ab100558) was not validated specifically with BAL samples - only with serum, plasma and cell culture supernatants. As long as the BAL samples have detectable level of IL17A Receptor, I would say  it will most likely work with BAL samples.  Although since we haven’t  tested this kit with this sample type, I cannot say with certainty. You may wish to conduct a search using PubMed  to see what types of samples can be used for detecting this target. The website of PubMed: http://www.ncbi.nlm.nih.gov/pubmed/ The price of this kit is: £335.00. I would suggest that you take a look at the on-line datasheet as well as the Protocol Booklet for further information: https://www.abcam.com/ps/products/100/ab100558/documents/IL17A-Receptor-Human-ELISA-Kit-ab100558-protocol-plain.pdf If you need any further assistance in the future, please do not hesitate to contact me.    

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