Overview

  • Product name

    Human IL-17E ELISA Kit (IL-25)
    See all IL-25 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 621pg/ml 24.2 3.9%
    2 16 1969pg/ml 90.6 4.6%
    3 16 2795pg/ml 150.9 5.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 730pg/ml 30.7 4.2%
    2 24 2103pg/ml 119.9 5.7%
    3 24 3065pg/ml 208.4 6.8%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human IL-17E Enzyme-Linked Immunosorbent Assay (ELISA) kit (IL-25) (ab213793) is designed for the quantitative detection of Human IL-17E in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-17E has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: Y33-G177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-17E amount of sample captured in plate.

  • Notes

    Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-κB activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human IL-17E coated Microplate (12 x 8 wells)  1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human IL-17E antibody 1 x 130µl
    Lyophilized recombinant Human IL-17E standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
  • Tissue specificity

    Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • IL 17E
    • IL 25
    • IL-17E
    • IL-25
    • IL17e
    • IL25
    • IL25_HUMAN
    • Interleukin 17E
    • Interleukin 25
    • Interleukin-17E
    • Interleukin-25
    • Interleukin17E
    • Interleukin25
    • OTTHUMP00000027946
    • OTTHUMP00000246238
    • UNQ3120/PRO10272
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IL-17E ELISA Kit (IL-25) (ab213793) Standard Curve

Protocols

References

ab213793 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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