Overview

  • Product name

    Human IL-17F ELISA Kit
    See all IL-17F kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    13.72 pg/ml - 10000 pg/ml
  • Recovery

    93.5 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 90.63 81% - 99%
    Serum 95.7 87% - 103%
    Plasma 94.25 81% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-17F Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17F in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-17F coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17F present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17F antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17F bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100557

Protocols

References

ab100557 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. 1. Here is a link to a kit that will detect human IL17F: https://www.abcam.com/Human-IL17F-ELISA-Kit-1-x-96-Well-Plate-ab100557.html Tested samples: Cell culture supernatant, Urine, Serum, Plasma Cost: £335.00 2. Here is a link to a kit that will detect human IL17A: https://www.abcam.com/IL17A-Receptor-Human-ELISA-Kit-1-x-96-Well-Plate-ab100558.html Tested samples: Cell culture supernatant, Urine, Serum, Plasma Cost: £335.00 3. Unfortunately we do not have an ELISA kit for detection of IL25.  The additional information you requested is the same as I noted in my previous email. I hope this is helpful. Please contact me again if you have any further questions.

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