Overview

  • Product name

    Human IL-17F ELISA Kit
    See all IL-17F kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 7.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 6.5%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    15.5 pg/ml
  • Range

    31.3 pg/ml - 2000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-17F Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-17F concentrations in Cell culture supernatant, serum and plasma (citrate, heparin).


    IL-17F specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an Biotin-conjugated anti-Human IL-17F detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17F captured on the plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119537.

Protocols

References

ab119537 has not yet been referenced specifically in any publications.

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