Overview

  • Product name

    Human IL-17RC ELISA Kit
    See all IL-17RC kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.8ng/ml 0.101 5.6%
    2 16 5ng/ml 0.32 6.4%
    3 16 9.7ng/ml 0.427 4.4%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    250 pg/ml - 16000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-17RC ELISA Kit (ab213794) is designed for the quantitative measurement of Human IL-17RC in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-17RC has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: L21-A454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-17RC amount of sample captured in plate. 

  • Notes

    Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human IL-17RC coated Microplate (12 x 8 wells)  1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human IL-17RC antibody 1 x 130µl
    Lyophilized recombinant Human IL-17RC standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Relevance

    IL-17RC is a single pass transmembrane protein that shares limited similarity with the interleukin 17 receptor. It is widely expressed in non leukocyte tissues and forms a heteromeric receptor for IL-17 with IL-17RA. At least four different isoforms are known to exist.
  • Cellular localization

    Cell membrane; Single pass type I membrane protein. Note: soluble isoforms may be produced.
  • Alternative names

    • IL 17 receptor C
    • IL 17RC
    • IL 17RL
    • IL17 receptor C
    • IL17F receptor
    • IL17RC
    • IL17RL
    • Interleukin 17 receptor C
    • Interleukin 17 receptor homolog
    • Interleukin 17 receptor like
    • Interleukin 17 receptor like protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IL-17RC ELISA Kit (ab213794) Standard Curve

Protocols

References

ab213794 has not yet been referenced specifically in any publications.

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