Human IL-18 R beta ELISA Kit (ab113325)
- Datasheet
- References
- Protocols
Overview
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Product name
Human IL-18 R beta ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 12 pg/ml -
Range
12.3 pg/ml - 3000 pg/ml -
Recovery
77 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 75.78 68% - 85% Serum 80.9 71% - 89% Plasma 74.98 67% - 83% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-18 R beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)
This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Human IL-18 R beta 2 vials IL-18 R beta Microplate 1 unit Recombinant Human IL-18 R beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. -
Tissue specificity
Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- IL18RAP
- Accessory protein like
- Accessory protein-like
see all -
Database links
- Entrez Gene: 8807 Human
- Omim: 604509 Human
- SwissProt: O95256 Human
- Unigene: 158315 Human
Images
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Representative Standard CurveRepresentative Standard Curve using ab113325
Protocols
References
ab113325 has not yet been referenced specifically in any publications.
