For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    human-il-18-r-beta-elisa-kit-ab113325.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email

Human IL-18 R beta ELISA Kit (ab113325)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Representative Standard Curve

    Key features and details

    • Sensitivity: 12 pg/ml
    • Range: 12.3 pg/ml - 3000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    You may also be interested in

    Protein
    Recombinant Human IL-18R Beta/IL-18RAP protein (ab191972)

    View more associated products

    Overview

    • Product name

      Human IL-18 R beta ELISA Kit
    • Detection method

      Colorimetric
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 12 pg/ml
    • Range

      12.3 pg/ml - 3000 pg/ml
    • Recovery

      77 %

      Sample specific recovery
      Sample type Average % Range
      Cell culture supernatant 75.78 68% - 85%
      Serum 80.9 71% - 89%
      Plasma 74.98 67% - 83%
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s IL-18 R beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)


      This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    • Notes

      Optimization may be required with urine samples.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 25ml
      500X HRP-Streptavidin Concentrate 1 x 200µl
      5X Assay Diluent B 1 x 15ml
      5X Assay Diluent D 1 x 15ml
      Biotinylated anti-Human IL-18 R beta 2 vials
      IL-18 R beta Microplate 1 unit
      Recombinant Human IL-18 R beta Standard (lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Interleukin ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
    • Function

      Required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex (By similarity). Together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK.
    • Tissue specificity

      Strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon, but not in any other tissue tested. Strongly expressed in T-cells polarized with IL-12. Specifically coexpressed with IL18R1 in Th1 cells.
    • Sequence similarities

      Belongs to the interleukin-1 receptor family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 TIR domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession O95256 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • IL18RAP
      • Accessory protein like
      • Accessory protein-like
      • AcPL
      • CD218 antigen-like family member B
      • CD218b
      • CDw218b
      • I18RA_HUMAN
      • IL 18RAcP
      • IL 1R accessory protein like
      • IL 1R7
      • IL 1RAcPL
      • IL-18 receptor accessory protein
      • IL-18R-beta
      • IL-18RAcP
      • IL-18Rbeta
      • IL-1R accessory protein-like
      • IL-1R-7
      • IL-1R7
      • IL-1RAcPL
      • IL18R Beta
      • IL18RAP
      • IL18Rbeta
      • Interleukin 18 receptor accessory protein
      • Interleukin-1 receptor 7
      • Interleukin-18 receptor accessory protein
      • Interleukin-18 receptor accessory protein-like
      • Interleukin-18 receptor beta
      • Interleukin-18 receptor beta chain
      see all
    • Database links

      • Entrez Gene: 8807 Human
      • Omim: 604509 Human
      • SwissProt: O95256 Human
      • Unigene: 158315 Human

      Associated products

        Images

        • Representative Standard Curve
          Representative Standard Curve

          Representative Standard Curve using ab113325

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (0)

        Publishing research using ab113325? Please let us know so that we can cite the reference in this datasheet.

        ab113325 has not yet been referenced specifically in any publications.

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        There are currently no Customer reviews or Questions for ab113325.
        Please use the links above to contact us or submit feedback about this product.

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
        For licensing inquiries, please contact partnerships@abcam.com

        Get resources and offers direct to your inbox Sign up
        A-Z by research area
        • Cancer
        • Cardiovascular
        • Cell biology
        • Developmental biology
        • Epigenetics & Nuclear signaling
        • Immunology
        • Metabolism
        • Microbiology
        • Neuroscience
        • Signal transduction
        • Stem cells
        A-Z by product type
        • Primary antibodies
        • Secondary antibodies
        • Biochemicals
        • Isotype controls
        • Flow cytometry multi-color selector
        • Kits
        • Loading controls
        • Lysates
        • Peptides
        • Proteins
        • Slides
        • Tags and cell markers
        • Tools & Reagents
        Help & support
        • Support
        • Make an Inquiry
        • Protocols & troubleshooting
        • Placing an order
        • RabMAb products
        • Biochemical product FAQs
        • Training
        • Browse by Target
        Company
        • Corporate site
        • Investor relations
        • Company news
        • Careers
        • About us
        • Blog
        Events
        • Tradeshows
        • Conferences
        International websites
        • abcam.cn
        • abcam.co.jp

        Join with us

        • LinkedIn
        • facebook
        • Twitter
        • YouTube
        • Terms of sale
        • Website terms of use
        • Cookie policy
        • Privacy policy
        • Legal
        • Modern slavery statement
        © 1998-2021 Abcam plc. All rights reserved.