Overview

  • Product name

    Human IL-18BP ELISA Kit
    See all IL-18BP kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    24.69 pg/ml - 18000 pg/ml
  • Recovery

    91 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106.7 96% - 121%
    Serum 83.5 74% - 97%
    Plasma 82.3 72% - 96%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-18BP (Interleukin-18 Binding Protein) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18BP in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-18BP coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100559

  • Representative standard curve using ab100559

Protocols

References

This product has been referenced in:

  • Nawaz SS  et al. Potential role of serum fetuin-A in relation with pro-inflammatory, chemokine and adhesion molecules in diabetic kidney disease: a case-control study. Mol Biol Rep 46:1239-1246 (2019). Read more (PubMed: 30632070) »
  • Menale C  et al. Bisphenol A effects on gene expression in adipocytes from children: association with metabolic disorders. J Mol Endocrinol 54:289-303 (2015). Read more (PubMed: 25878060) »
See all 3 Publications for this product

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