Overview

  • Product name

    Human IL-18BP ELISA Kit
    See all IL-18BP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3.5%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.3%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.9 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 94 87% - 102%
    Serum 115 103% - 122%
    Cell culture media 103 99% - 108%
    Heparin Plasma 90 74% - 102%
    EDTA Plasma 86 78% - 90%
    Citrate Plasma 86 80% - 90%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkeyDoes not react with: Mouse, Rat, Cow
  • Product overview

    IL-18BP in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-18BP in Human serum, plasma, urine, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab224877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL in neat serum, 2682 pg/mL in neat plasma (citrate), 3360 pg/ml in neat plasma (heparin), 2957 pg/mL in neat plasma (EDTA), and 11,400 pg/ml in neat urine.

  • The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: THP-1 supernatant 100% and RPMI 1640 Media (containing 10% fetal bovine serum) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL with a range of 3081 – 6405 pg/mL.

Protocols

References

ab224877 has not yet been referenced specifically in any publications.

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