Overview

  • Product name

    Human IL-19 ELISA Kit
    See all IL-19 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Milk 8 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Milk 3 3%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.648 pg/ml
  • Range

    7.81 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 109 103% - 113%
    Saliva 106 105% - 108%
    Milk 100 98% - 103%
    Urine 83 81% - 86%
    Serum 89 86% - 90%
    Heparin Plasma 89 88% - 92%
    EDTA Plasma 87 87% - 88%
    Citrate Plasma 86 86% - 88%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IL-19 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-19 protein in human serum, plasma, urine, saliva, breast milk, and supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Human Interleukin 19 (IL-19) belongs to the IL-10 family of related cytokines. IL-19 comprised of seven helices and is secreted as a 35 kDa glycosylated monomer. IL-19 expression has been observed in keratinocytes and monocytes. IL-19 binds a receptor complex consisting of IL-20R alpha and the IL-20 R beta. This receptor complex is also shared by IL-20 and IL-24. IL-19 has been associated with some important roles in inflammatory response such as up-regulation of IL-6 and TNF-alpha and induces apoptosis.


     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-19 Capture Antibody 1 x 600µl
    10X Human IL-19 Detector Antibody 1 x 600µl
    Human IL-19 Lyophilized Recombinant Protein 2 vials
    Antibody Diluent 4BI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Sample Diluent NS 1 x 50ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
  • Research areas

  • Function

    May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • IL 10C
    • IL 19
    • IL-19
    • Il19
    • IL19_HUMAN
    • Interleukin 19
    • Interleukin-19
    • MDA1
    • Melanoma differentiation-associated protein-like protein
    • NG.1
    • ZMDA1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab231922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between Human IL-19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • The IL-19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-19 were measured in duplicate, interpolated from the IL-19 standard curves.

  • The concentrations of IL-19 were measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 37.14 ng/mL in milk, 91.95 pg/mL in urine, 779.30 pg/mL in saliva, and 1008.97 pg/mL in PBMC supernatant.

  • The concentrations of IL-19 was measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 91.95 pg/mL in urine.

Protocols

References

ab231922 has not yet been referenced specifically in any publications.

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