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We need to study human intracellular IL-1 alpha ELISA and plan to order your product ab178008. Would you please tell us can this product detect all form IL-1 alpha, including pro- and mature-IL-1 alpha?
Asked on Mar 10 2015
Thank you for contacting us.
Only the cleaved/mature form is secreted, therefore we are confident that the assay measures cleaved IL-1a as we can measure levels of IL1a in stimulated PBMCs in comparison to the non-stimulated PBMCs supernatant. We have not tested whether the assay measures non-cleaved IL1a.
Heather AllenAbcam Scientific Support
Answered on Mar 10 2015