Product nameHuman IL-1ra Matched Antibody Pair Kit
See all IL-1RA kits
Assay typeELISA set
Range7.8 pg/ml - 500 pg/ml
Species reactivityReacts with: Human
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-1ra. Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab211650) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Human IL-1ra Capture Antibody 1 x 100µg 1 x 20µg Human IL-1ra Detector Antibody 1 x 25µg 1 x 5µg Human IL-1ra Lyophilized Protein 1 vial 1 vial
FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in diseaseMicrovascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency
Cellular localizationCytoplasm and Secreted.
- Information by UniProt
- ICIL 1RA
Our Abpromise guarantee covers the use of ab210888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab210888 has not yet been referenced specifically in any publications.