Overview

  • Product name

    Human IL-2 ELISA Kit
    See all IL-2 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 4 pg/ml
  • Range

    2.07 pg/ml - 1500 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.27 84% - 103%
    Plasma 95.52 83% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-2 ELISA kit is designed for the quantitative measurement of Human IL-2 in plasma, and cell culture supernatants. (Human IL-2 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-2 in normal serum with ab100566, only in serum spiked with Human IL-2.


    Get results in 90 minutes with Human IL-2 ELISA Kit (ab174444) from our SimpleStep ELISA® range.


     

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100566

  • Representative standard curve using ab100566

Protocols

References

ab100566 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100566 IL2 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-2 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100570 IL4 Human ELISA Kit
o Capture Antibody
§ Mouse, monoclonal
§ Not tested for cross-reactivity with mouse samples.
o Detection Antibody
§ Goat, polyclonal
§ Detects the region His25­Ser153 (Accession # NP_000580)
§ Not tested for cross-reactivity with mouse samples.
· ab100689 Interferon gamma Mouse ELISA Kit
o Capture Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
· ab100697 IL10 Mouse ELISA Kit
o Capture Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
· ab100706 IL2 Mouse ELISA Kit
o The detection antibody did not show any cross-reactivity with Human IL-2 from testing. Thus, this kit is not expected to cross react with human samples.
· ab100710 IL4 Mouse ELISA Kit
o The capture antibody did not show any cross-reactivity with Human IL-4 from testing. Thus, this kit is not expected to cross react with human samples.

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Answer

Thank you for contacting us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am sorry to hear you and Anneli have been experiencing problems with the IL2 Human ELISA Kit ab100566. I have arranged a replacement of the HRP Streptavidin Concentrate for you with the order number xxxxx. To check the status of this shipment please contact our Customer Service team and reference this number. Also, I am a little bit confused why you contacted VWR. According to our records, neither the order nor the shipment was linked to VWR. I am very sorry that the advice you were given by them was not helpful at all. May I suggest that you contact our technical service directly in case you encounter any difficulties with our products in the future? We are happy to offer any technical support, and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund - regardless how it was ordered (directly from us or through a distributor). I apologize for the inconvenience this has caused and hope this experience will not prevent you from purchasing other products from us in the future. Please do not hesitate to contact us if you have any further questions or concerns.

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