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Abcam’s IL-2 (Interleukin-2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in supernatants, buffered soloutions, serum and plasma samples.
A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-2.
Get results in 90 minutes with Human IL-2 ELISA Kit (ab174444) from our SimpleStep ELISA® range.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated anti IL-2||Red||1 x 400µl||2 x 400µl|
|Biotinylated Antibody Diluent||Red||1 x 7ml||1 x 13ml|
|TMB Substrate||1 x 11ml||1 x 24ml|
|Control||Silver||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|IL-2 Microplate (12 x 8 well strips)||1 unit||2 units|
|IL-2 Standard (Lyophilized)||Yellow||2 vials||4 vials|
|Standard Diluent (Human Serum)||1 x 7ml||2 x 7ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||1 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab46033 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"