• Product name

    Human IL-2 Receptor ELISA Kit
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Overall 4.5%
    Sample n Mean SD CV%
    1 6 1594pg/ml 184 11.6%
    2 6 1528pg/ml 135 8.8%
    3 6 1705pg/ml 204 12%
    4 6 1124pg/ml 171 15.2%
    5 6 2305pg/ml 165 7.2%
    6 6 1824pg/ml 126 69%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    32.5 pg/ml
  • Range

    68.75 pg/ml - 2220 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Serum 99 86% - 122%

  • Assay time

    3h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-2 Receptor Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 Receptor in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Human IL-2 Receptor.

    A monoclonal antibody specific for IL-2 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 Receptor present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-2 Receptor.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-2 Receptor Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-2 Receptor Control Silver 2 vials 4 vials
    IL-2 Receptor Microplate (12 x 8 well strips) 1 unit 2 units
    Plastic Plate Covers 2 units 4 units
    Stop Reagent Black 1 x 11ml 1 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Relevance

    The IL-2 Receptor, which is involved in T cell-mediated immune responses, alpha (IL-2RA) and beta (IL-2RB) chains, together with the common gamma chain (IL-2RG), constitute the high-affinity IL-2 receptor. Homodimeric alpha chains (IL-2RA) result in low-affinity receptor. Normally an integral-membrane protein, soluble IL-2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL-2RA mRNAs have been isolated, but the significance of each is presently unknown. Homodimeric beta (IL-2RB) chains produce a medium-affinity receptor. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The interleukin 2 (IL-2) receptor gamma chain (IL-2RG), an important signalling component of many interleukin receptors (IL-2,IL-4,IL-7,IL-9, and IL-15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked gene cause X-linked severe combined immunodeficiency (XSCID).
  • Cellular localization

    Cell Membrane; single-pass type I membrane protein.
  • Alternative names

    • CD122
    • CD122 antigen
    • CD132
    • CD132 antigen
    • CD25
    • CD25 antigen
    • Cytokine receptor common gamma chain
    • Gamma C
    • High affinity IL 2 receptor subunit beta
    • IDDM10
    • IL 2 receptor
    • IL 2 receptor alpha subunit
    • IL 2R gamma chain
    • IL-2-RA
    • IL2 Receptor
    • IL2-RA
    • IL2R
    • IL2RA
    • IL2RB
    • IL2RG
    • IMD4
    • interleukin 2 receptor alpha
    • Interleukin 2 receptor alpha chain
    • interleukin 2 receptor beta
    • Interleukin 2 receptor beta chain
    • interleukin 2 receptor gamma
    • Interleukin 2 receptor gamma chain
    • p55
    • p64
    • P70 75
    • p75
    • SCIDX
    • SCIDX1
    • TAC antigen
    • TCGFR
    • XSCID
    see all
  • Database links


Our Abpromise guarantee covers the use of ab46036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab46036



This product has been referenced in:

  • Meng D  et al. Effects of VEGFR1+ hematopoietic progenitor cells on pre-metastatic niche formation and in vivo metastasis of breast cancer cells. J Cancer Res Clin Oncol 145:411-427 (2019). Read more (PubMed: 30483898) »
  • Ngcobo M & Gqaleni N Evaluation of the immunomodulatory effects of a South African commercial traditional immune booster in human peripheral blood mononuclear cells. BMC Complement Altern Med 16:300 (2016). Read more (PubMed: 27550057) »
See all 2 Publications for this product

Customer reviews and Q&As


Thank you for contacting us. The two soluble IL2 receptor ELISA kits, ab46036 (with plate) and ab47354 (capture and detection antibodies sufficient for five plates, but plates are not included) are known to be reactive with the alpha sub-unit. Reactivity with the beta and gamma subunits has not been tested. Click here (or use the following: https://www.abcam.com/index.html?datasheet=47354). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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