Human IL-20 ELISA Kit (ab171344)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameHuman IL-20 ELISA Kit
See all IL-20 kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample typeCell culture supernatant, Serum, Heparin Plasma, EDTA Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 10 pg/ml
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Range62.5 pg/ml - 4000 pg/ml
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s Human IL-20 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human IL-20 in cell culture supernatants, serum and plasma (heparin, EDTA).
An IL-20 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for IL-20 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human IL-20 amount of sample captured in plate.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human IL-20 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-Human IL-20 antibody 1 x 130µl Lyophilized recombinant Human IL-20 standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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FunctionCytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.
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Tissue specificityExpressed at very low levels in skin, trachea, and other tissues.
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Sequence similaritiesBelongs to the IL-10 family.
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Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- Cytokine Zcyto10
- Four alpha helix cytokine
- IL-20
see all -
Database links
- Entrez Gene: 50604 Human
- Omim: 605619 Human
- SwissProt: Q9NYY1 Human
- Unigene: 272373 Human
Applications
Our Abpromise guarantee covers the use of ab171344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical standard curveRepresentative standard curve using ab171344.
Protocols
References
This product has been referenced in:
- Tamassia N et al. A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It. Front Immunol 9:795 (2018). Read more (PubMed: 29719541) »
