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    human-il-20-elisa-kit-ab171344.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Human IL-20 ELISA Kit (ab171344)

  • Datasheet
  • SDS
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Typical standard curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 62.5 pg/ml - 4000 pg/ml
    • Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Protein
    Recombinant human IL-20 protein (ab201402)

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    Overview

    • Product name

      Human IL-20 ELISA Kit
      See all IL-20 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%
    • Sample type

      Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      62.5 pg/ml - 4000 pg/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Human IL-20 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human IL-20 in cell culture supernatants, serum and plasma (heparin, EDTA). 


      An IL-20 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for IL-20 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human IL-20 amount of sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human IL-20 antibody Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human IL-20 antibody 1 x 130µl
      Lyophilized recombinant Human IL-20 standard 2 x 10ng
      Plate Seal 4 units
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Amber Bottle 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interleukins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
    • Function

      Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.
    • Tissue specificity

      Expressed at very low levels in skin, trachea, and other tissues.
    • Sequence similarities

      Belongs to the IL-10 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q9NYY1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Cytokine Zcyto10
      • Four alpha helix cytokine
      • IL-20
      • IL10D
      • IL20
      • IL20_HUMAN
      • Interleukin 20
      • Interleukin-20
      • MGC96907
      • UNQ852/PRO1801
      • ZCYTO10
      see all
    • Database links

      • Entrez Gene: 50604 Human
      • Omim: 605619 Human
      • SwissProt: Q9NYY1 Human
      • Unigene: 272373 Human

      Associated products

        Images

        • Typical standard curve
          Typical standard curve

          Representative standard curve using ab171344.

        Protocols

        • Protocol Booklet
        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (1)

        Publishing research using ab171344? Please let us know so that we can cite the reference in this datasheet.

        ab171344 has been referenced in 1 publication.

        • Tamassia N  et al. A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members In Vitro: Failure to Reproducibly Detect It. Front Immunol 9:795 (2018). PubMed: 29719541

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