Human IL-20 ELISA Kit (ab231927)
- Datasheet
- References
- Protocols
Overview
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Product name
Human IL-20 ELISA Kit
See all IL-20 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Spiked serum 5 3.2% Inter-assay Sample n Mean SD CV% Spiked serum 3 3.5% -
Sample type
Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.2 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 102% - 112% Cell culture media 109 105% - 111% Heparin Plasma 97 91% - 103% EDTA Plasma 93 89% - 97% Citrate Plasma 98 96% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
IL-20 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-20 protein in human serum, plasma, and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
IL-20 is a cytokine structurally related to IL-10. IL-20 is expressed in monocytes and keratinocytes and is upregulated during inflammation. IL-20 binds to IL-20R2 and IL-20R1 receptors forming complexes that can result in hyperproliferation of keratinocytes, and cause the onset of other inflammatory disease such as rheumatic arthritis, psoriasis, and atherosclerosis.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-20 Capture Antibody 1 x 600µl 10X Human IL-20 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-20 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. -
Tissue specificity
Expressed at very low levels in skin, trachea, and other tissues. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine Zcyto10
- Four alpha helix cytokine
- IL-20
see all -
Database links
- Entrez Gene: 50604 Human
- Omim: 605619 Human
- SwissProt: Q9NYY1 Human
- Unigene: 272373 Human
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab231927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human IL-20 ELISA Kit (ab231927)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-20 Competitor standard curve comparisonStandard curve comparison between Human IL-20 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human IL-20 standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked IL-20 in human serum and plasma samplesThe concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Serum from ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). No detectable levels of IL-20 were measured.
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Interpolated concentrations of spiked IL-20 in human cell culture media sampleThe concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Protocols
References
ab231927 has not yet been referenced specifically in any publications.
