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    human-il-20-elisa-kit-ab231927.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantSimpleStep

Human IL-20 ELISA Kit (ab231927)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human IL-20 ELISA Kit (ab231927)
  • Human IL-20 Competitor standard curve comparison
  • Example of human IL-20 standard curve
  • Interpolated concentrations of spiked IL-20 in human serum and plasma samples
  • Interpolated concentrations of spiked IL-20 in human cell culture media sample
  • Sandwich ELISA - Human IL-20 ELISA Kit (ab231927)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 4.2 pg/ml
  • Range: 7.8 pg/ml - 500 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

Protein
Recombinant human IL-20 protein (ab201402)

View more associated products

Overview

  • Product name

    Human IL-20 ELISA Kit
    See all IL-20 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Spiked serum 5 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Spiked serum 3 3.5%
  • Sample type

    Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.2 pg/ml
  • Range

    7.8 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 106 102% - 112%
    Cell culture media 109 105% - 111%
    Hep Plasma 97 91% - 103%
    EDTA Plasma 93 89% - 97%
    Cit plasma 98 96% - 107%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-20 ELISA Kit (ab231927) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-20 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-20 with 4.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-20 Capture Antibody 1 x 600µl
    10X Human IL-20 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human IL-20 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
  • Function

    Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.
  • Tissue specificity

    Expressed at very low levels in skin, trachea, and other tissues.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9NYY1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Cytokine Zcyto10
    • Four alpha helix cytokine
    • IL-20
    • IL10D
    • IL20
    • IL20_HUMAN
    • Interleukin 20
    • Interleukin-20
    • MGC96907
    • UNQ852/PRO1801
    • ZCYTO10
    see all
  • Database links

    • Entrez Gene: 50604 Human
    • Omim: 605619 Human
    • SwissProt: Q9NYY1 Human
    • Unigene: 272373 Human

    Associated products

    • Alternative Versions

      • Human IL-20 Antibody Pair - BSA and Azide free (ab244192)

    Images

    • Other - Human IL-20 ELISA Kit (ab231927)
      Other - Human IL-20 ELISA Kit (ab231927)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human IL-20 Competitor standard curve comparison
      Human IL-20 Competitor standard curve comparison

      Standard curve comparison between Human IL-20 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

    • Example of human IL-20 standard curve
      Example of human IL-20 standard curve

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spiked IL-20 in human serum and plasma samples
      Interpolated concentrations of spiked IL-20 in human serum and plasma samples

      The concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

      Serum from ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). No detectable levels of IL-20 were measured.

    • Interpolated concentrations of spiked IL-20 in human cell culture media sample
      Interpolated concentrations of spiked IL-20 in human cell culture media sample

      The concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Sandwich ELISA - Human IL-20 ELISA Kit (ab231927)
      Sandwich ELISA - Human IL-20 ELISA Kit (ab231927)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab231927? Please let us know so that we can cite the reference in this datasheet.

    ab231927 has not yet been referenced specifically in any publications.

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