Overview

  • Product name

    Human IL-22 ELISA Kit
    See all IL-22 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 2.6%
  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.61 pg/ml
  • Range

    13.12 pg/ml - 840 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101 98% - 104%
    Urine 107 101% - 111%
    Serum 102 100% - 104%
    Hep Plasma 102 99% - 104%
    EDTA Plasma 102 100% - 105%
    Cit plasma 93 91% - 97%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    IL-22 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-22 protein in human serum, plasma, cell culture supernatant and urine. (UniprotID: Q9GZX6)


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in Sample Diluent NS: 2.61 pg/mL


    Samples in Sample Diluent 50BS: 4.71 pg/mL

  • Notes

    Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-22 Capture Antibody 1 x 600µl
    10X Human IL-22 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human IL-22 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cytokine that contributes to the inflammatory response in vivo.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Cytokine Zcyto18
    • IL 10 related T cell derived inducible factor
    • IL 21
    • IL 22
    • IL D110
    • IL TIF
    • IL-10-related T-cell-derived-inducible factor
    • IL-22
    • IL-TIF
    • IL21
    • Il22
    • IL22_HUMAN
    • ILD110
    • ILTIF
    • Interleukin 10 related T cell derived inducible factor
    • interleukin 21
    • Interleukin 22
    • Interleukin-22
    • MGC79382
    • MGC79384
    • TIFa
    • TIFIL 23
    • TIFIL23
    • UNQ3099/PRO10096
    • zcyto18
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab216170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1015 pg/mL in PBMC supernatant.

  • The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1174 pg/mL in serum, 1099 pg/mL in plasma (citrate), 1197 pg/mL in plasma (heparin), 1189 pg/mL in plasma (EDTA) and 1,322 pg/mL in urine.

  • Conditioned media was harvested after 48 hours. IL-22 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-22 were measured in duplicate, interpolated from the IL-22 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 316 pg/mL in PHA stimulated PBMC supernatant and 93 pg/mL in unstimulated supernatant.

Protocols

References

ab216170 has not yet been referenced specifically in any publications.

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