Overview

  • Product name

    Human IL-23 ELISA Kit
    See all IL-23 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2.7%
  • Sample type

    Serum, Cell culture media, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    15.6 pg/ml
  • Range

    39.1 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 113 108% - 120%
    Cell culture media 118 114% - 124%
    Heparin Plasma 83 80% - 86%
    EDTA Plasma 113 110% - 116%
    Citrate Plasma 107 103% - 110%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IL-23 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-23 protein in serum, plasma and cell culture supernatant. 


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     

  • Notes

    IL-23 acts as a growth factor for activated T and NK cells.  IL-23 induces auto immune inflammation and may be responsible for auto immune inflammatory diseases.  IL-23 is a complexed heterodimer of the IL-23A (19 subunit) and IL-12B (p40 subunit).  The ab221837 pair recognize the heterodimer by using antibodies to both the p19 and p40 subunits.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-23 Capture Antibody 1 x 600µl
    10X Human IL-23 Detector Antibody 1 x 600µl
    Human IL-23 Lyophilized Recombinant Protein 1 x 2 vials
    Antibody Diluent 4BI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificity

    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Developmental stage

    Expressed by newborns dendritic cells.
  • Cellular localization

    Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • Alternative names

    • IL 23
    • IL 23 A
    • IL 23 subunit alpha
    • IL 23A
    • IL 23p19
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • IL12B
    • IL23
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • interleukin 12B
    • Interleukin 23 alpha subunit p19
    • Interleukin 23 p19 subunit
    • interleukin 23 subunit alpha
    • interleukin 23 subunit p19
    • interleukin six, G CSF related factor
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • MGC79388
    • P19
    • SGRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab221837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: DMSO and LPS stimulated THP-1 cell culture supernatant 100% and IFN-γ and LPS stimulated THP-1 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23 concentration was determined to be 346 pg/mL in DMSO and LPS stimulated THP-1 cell culture supernatant and 834 pg/mL in IFN-γ and LPS stimulated THP-1 cell culture supernatant. Media and unstimulated THP-1 cell culture supernatant were undetectable.

Protocols

References

ab221837 has not yet been referenced specifically in any publications.

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