Overview

  • Product name

    Human IL-23 ELISA Kit
    See all IL-23 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    A 6 4637pg/ml 72 1.6%
    B 6 3908pg/ml 36 1%
    C 6 3016pg/ml 72 2.4%
    Inter-assay
    Sample n Mean SD CV%
    A 9 4439pg/ml 276 6.2%
    B 9 3807pg/ml 353 9.2%
    C 9 2570pg/ml 388 15%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    156.2 pg/ml - 5000 pg/ml
  • Recovery

    89 %

    Sample specific recovery
    Sample type Average % Range
    Serum 118 103% - 134%

  • Assay time

    2h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-23 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


    A monoclonal antibody specific for IL-23 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-23 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-23 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-23 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-23.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-23 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-23 Microplate(12 x 8 well strips) 1 unit 2 units
    IL-23 Standard (Lyophilized) Yellow 2 vials 4 vials
    Plastic Plate Covers 2 units 4 units
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Function

    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificity

    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Developmental stage

    Expressed by newborns dendritic cells.
  • Cellular localization

    Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • Alternative names

    • IL 23
    • IL 23A
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • IL23
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • Interleukin 23 alpha subunit p19
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • P19
    • SGRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab64708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  •  

    Representative Standard Curve using ab64708

Protocols

References

This product has been referenced in:

  • Yu X  et al. Intensive Cytokine induction in Pandemic H1N1 Influenza Virus Infection Accompanied by Robust Production of IL-10 and IL-6. PLoS One 6:e28680 (2011). ELISA . Read more (PubMed: 22174866) »
  • Abe T  et al. Combination use of immune complexes and a Ca2(+) channel blocker azelnidipine enhances interleukin-12 p40 secretion without T helper type 17 cytokine secretion in human monocyte-derived dendritic cells. Clin Exp Immunol 156:405-12 (2009). ELISA . Read more (PubMed: 19438591) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Q&A

Answer

Thank you for contacting us.

The second antibody used in kit is now published. Please check datasheet of ab136544.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your email.

I apologise for confusion. This kit ab64708 has two mouse monoclonal antibodies in it, one is clone B-F43 and other is B-Z23. The clone B-Z23 is available for purchasing with abID 84471. The clone B-F43 is not yet available however we are in process of publishing it. I will confirm the catalogue number once it gets published.

You can buy both antibodies for developing your own ELISA.

The antibodies in this kit bind to p19 subunit, they do not bind to p40.

I hope this information will be helpful. Should you have any further question please do not hesitate to contact me.

Read More

Answer

Thank you for contacting us.

The antibody ab84471 which is one of antibody in kit binds to subunit p19.

I have ask the datasheet team to add clone B-F43 in catalogue which is the second antibody in the kit. I will let you know the catalogue number soon.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your interest in the MMP-3 and IL23 kits.

The volume of sample required for each test is 100ul per well of the assay plate.

Regarding the 100% discount, I want to be sure you understand the offer, which is also described at the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

The discount is not applied to the purchase of the kits. The kits are purchased full-price after we issue you a code. After you test the kits on the monkey serum, you then send Abcam a review of your results (with the code ID in the review), via our Abreview form. ( Please see www.abcam.com/abreviews).

After we receive your reviews, the code is then "active" and can be redeemed for a free kit. Or, it can be used to receive a 20% discount on a primary antibody or a protein.

In addition, 440 points will be awarded for the first review published for ELISA products. Please see https://www.abcam.com/index.html?pageconfig=resource&rid=14713 for more details.

These offers are available through June.

Please let me know if you are still interested, and I will send you the codes, which are necessary before you place your order.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up